Geminiviruses Subvert Ubiquitination by Altering CSN-Mediated Derubylation of SCF E3 Ligase Complexes and Inhibit Jasmonate Signaling in Arabidopsis thaliana

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Geminiviruses subvert ubiquitination by altering CSN-mediated derubylation of SCF E3 ligase complexes and inhibit jasmonate signaling in Arabidopsis thaliana.

Viruses must create a suitable cell environment and elude defense mechanisms, which likely involves interactions with host proteins and subsequent interference with or usurpation of cellular machinery. Here, we describe a novel strategy used by plant DNA viruses (Geminiviruses) to redirect ubiquitination by interfering with the activity of the CSN (COP9 signalosome) complex. We show that gemini...

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Characterization of the Arabidopsis thaliana E3 Ubiquitin-Ligase AtSINAL7 and Identification of the Ubiquitination Sites

Protein ubiquitination leading to degradation by the proteasome is an important mechanism in regulating key cellular functions. Protein ubiquitination is carried out by a three step process involving ubiquitin (Ub) activation by a E1 enzyme, the transfer of Ub to a protein E2, finally an ubiquitin ligase E3 catalyzes the transfer of the Ub peptide to an acceptor protein. The E3 component is res...

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The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis.

Xie and colleagues previously isolated the Arabidopsis COI1 gene that is required for response to jasmonates (JAs), which regulate root growth, pollen fertility, wound healing, and defense against insects and pathogens. In this study, we demonstrate that COI1 associates physically with AtCUL1, AtRbx1, and either of the Arabidopsis Skp1-like proteins ASK1 or ASK2 to assemble ubiquitin-ligase com...

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The Arabidopsis CSN5A and CSN5B subunits are present in distinct COP9 signalosome complexes, and mutations in their JAMM domains exhibit differential dominant negative effects on development.

The COP9 signalosome (CSN) is an evolutionarily conserved multisubunit protein complex involved in a variety of signaling and developmental processes through the regulation of protein ubiquitination and degradation. A known biochemical role attributed to CSN is a metalloprotease activity responsible for the derubylation of cullins, core components for several types of ubiquitin E3 ligases. The ...

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Ubiquitin-Ligase Complexes Are Required for Jasmonate Response in Arabidopsis

a Institute of Biotechnology Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China b Institute of Molecular Agrobiology, 1 Research Link, National University of Singapore, 117604, Republic of Singapore c Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, 12 Rue du Général Zimmer, 67084 Strasbourg Cédex, France d Plant Biotechnology ...

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ژورنال

عنوان ژورنال: The Plant Cell

سال: 2011

ISSN: 1532-298X,1040-4651

DOI: 10.1105/tpc.110.080267